DNA
ArfB

Part:BBa_K1360000:Design

Designed by: Jiang Liang   Group: iGEM14_Tongji   (2014-10-07)


alpha-L-arabinofuranosidase ArfB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 679
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1156
    Illegal BsaI.rc site found at 1282
    Illegal BsaI.rc site found at 1372


Design Notes

codon optimized for E.coli


Source

from whole genome sequenceing of Clostridium stercorarium DSM8532, codon optimized for E.coli

References